CONSTRUCTION OF THE RECOMBINANT PLASMIDS CARYING BamHI C FRAGMENT OF HERPES SIMPLEX VIRUS I AND INSERTED THYMIDIN KINASE GENE-MINI Mu PHAGE DNA
- VernacularTitle:构建由胸腺嘧啶激酶基因—小Mu噬菌体DNA插入到HSV BamHI C片段的重组质粒
- Author:
Yonglie CHU
- Publication Type:Journal Article
- Keywords:
herpes simplex virus;
Mu phage;
genetic recombination;
radom insertion
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
In order to mutate the BamHI C fragment of Herpes Simplex Virus 1 (HSV-1), the thymidin kinase gent-mini Mu phage DNA (TK-mM) was randomly inserted into the BamHI C fragment in the plasmid pRB 132 with DNA recombination technique. The six recombinant plasmids carrying the BamHI C fragment of HSV-1 and inserted TK-mM were isolated with DNA hybridization. The results of restriction endonucleasc analysis showed that the BamHI C fragment has been mutated by insertion of TK-mM system, and the insertional sites of TK-mM located in the six recombinant plasmids were different.
