Identification of glycoprotein C of herpes simplex virus
- VernacularTitle:放射免疫沉淀试验鉴定单纯疱疹病毒糖蛋白C
- Author:
Qigui YU
- Publication Type:Journal Article
- Keywords:
Anti-HSV McAb;
Glycoprotein;
Target antigen;
ELISA blocking test;
Radioprecipitational test
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
A group of glycoproteins with ⅥW 105- 130 K D from BHK-21 cell infected with Herpes Simplex Virus (HSV) were precipitated by six monoclonal antibodies (McAb). 1 A 12. Mad-2, 2D11, CM-D3, 2A8 and lC4,by using immunoprecipate technique. Im munoassays of BHK-21 cells infected with HSV-1/HSV-2 intertypic recom binants localized the genes encoding the target antigens of 1 A12, Mad-2, 2Dll, CM-D3, 2A8 and 1 C4 to the unique long region of HSV genesome at mapping unit 0.536—0.682 overlapping the gene encoding glycoprotein C(gC). De pending on reactivity with HSV- 1 and HSV-2 infected BHK-21 cells, Mad-2 shows HSV type-1 specificity, C M-D 3 shows HSV ty pe-2 specificity and the other four McAbs show type-common spe. cifity. So Mad-2 is against gC-1, CM D3 is against gC - 2 and 1 A12, 2 A8, 2 D11, 1 C4 are against type-common gC. We labelled the four McAbs directed at type-common gC with horseradise peroxidase (HRP)by the modified method of Nakane. ELISA blocking test was done to test the relationships of them. The result is that those four McAbs are against four distinct antigenic sites on type-common gC.
