Construction and cytotoxicity of two-killing-agent immunotoxin containing Ricin and Adriamycin
- VernacularTitle:含有篦麻毒素和阿霉素的双弹头免疫毒素的构建及其杀伤活性
- Author:
Shusheng XIE
- Publication Type:Journal Article
- Keywords:
Immunotoxin;
Ricin;
Adriamycin
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
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Abstract:
The synergistic effect of combinations of killing agents on tumor cells has long been recognized. Fodstad et al, reported that the combinations of Ricin and Adriamycin have a strong, syn ergistic effect on the L1210 leukemia cells. In this report, a novel immunotoxin containing Ricin and Adriamycin, BDI-l-Ricin-Adriamycin, was described.Ricin and Adriamycin were chemically coupled onto one monoclonal antibody molecule against human bladder cancer, BDI-1, to construct the two-killing-agent containing immunotoxin. The results of indirect immunofluorescence test and competiton bindig assay showed that the retention of 70% of the antibody binding activity in this immunotoxin was obtained. The in vitro cytotoxicity assay indicated that the immunotoxin in the presence of 0.1M galactose is 30,000 times more cytotoxic than BDI-1-Adriamycin conjugate and 10 times more cytotoxic than BDI-1-Ricin immunotoxin in the bladder cancer-specific killing, but has no cytotoxic effect on the LOVO colon carcinoma cells. This two-killing-agent containing immunotoxin may lead to the development of a new kind of immunotoxins which have strong cytotoxic activity to target cells.
