HPLC-ECD assay central monoamine transmitters and kinetic change of central monoamine transmitters during immunological response
- VernacularTitle:HPLC-ECD法分析中枢单胺类递质及其在免疫应答过程中的动态变化
- Author:
Jingping YANG
;
Guizhen YANG
- Publication Type:Journal Article
- Keywords:
Reverse phase ion-pair HPLC;
Electrochemical detection;
Monoamine transmitters;
Immunological response
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
A rapid and simple chromatographic procedure using HPLC-ECD is described for simultaneousdetermination of NE. E. DA. 5-HT with their precursor amino acid (Tyr. Trp) and their main metabolites (HVA. 5-HIAA). Using this assay,eight substrates are measured in cortex, diencephalon and brain stem of mice duing immunological response challenged by SRB6 3 days after challenged, the DA HVA in cortex, NE in diencephalon decrease obviously (p
