STUDY ON THE EXPERIMENTAL CONDITIONS OF BMG-INDUCED CHONDROCYTE FORMATION IN VITRO
- VernacularTitle:体外诱导软骨细胞形成的实验方法研究
- Author:
Zengdun SHI
- Publication Type:Journal Article
- Keywords:
BMG;
fetal rat musele;
tissue culture;
chondrocyte
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Thigh skeletal muscle of 19d～21d(near-term) fetal rat cultured together with bone matrix gelatin (BMG) in vitro. The medium was DMEM containing 3700 mg/L NaCHO_3. After 1O d in culture, there were BMG-induced chondrocytes in 7 out of 8 explants. After 15、20 and 25 d in culture. BMG-induced chondrocytes were observed in all of the 30 expiants(10 explants in each point of time-period). The result shows that the experimental conditions and culture medium used in this paper were suitable for the system of BMG-induced choudrocyte formation in vitro.
