Purification and characterization of soluble interleukin 2 receptor
- VernacularTitle:可溶性IL-2R的纯化及特性研究
- Author:
Xun ZHU
;
Guizhen YANG
- Publication Type:Journal Article
- Keywords:
Interleukin 2;
Soluble Interleukin 2 Receptor
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
By using 5 G 1-sepharose 4 B immune affinity column, we recovered approximately 160 ?g purified and soluble IL-2 receptor (sIL-2 R ) from 500ml PHA-stimulated human PBL supernatant. Only one band was showed in SDS-PAGE, its molecular weight was about 44 Kd. The purified sIL-2 R, which blocked the binding of anti-IL-2 R monoclonal antibody with IL- 2 R~+ cell, inhibited (at the higher doses) but enhanced (at the lower doses) the growth of IL-2 dependent cells.
