A chemical method for preparing biotin-labeled nucleic acid probe
- VernacularTitle:化学法标记生物素核酸探针的实验研究
- Author:
Zhongbin LIU
;
Guizhen YANG
- Publication Type:Journal Article
- Keywords:
Biotin-Labeled Nucleic Acid Probe;
Chemical Labeling;
Hybridization
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
A chemical method to prepare biotin-labeled nucleic acid probe for nonisotopic hybridization is introduced. The method involves the bisulfite-catalyzed transamination reaction of cytosine residues in nucleic acids with ethylenediamine or hexanediamine. Primary amino groups on the cytosine derivatives are then reacted with biotiny-N-hydroxysuccinimide ester. Results of dot blot and Southern blot carried out with biotinlabeled ? DNA probe show that such nonisotopic probe method is sensitive, specific, repeatable and unexpensive.
