Isolation, identification and expression of a cDNA clone encoding human GM-CSF
- VernacularTitle:用负向选择增富法筛选人GM-CSF cDNA
- Author:
Ying SUN
- Publication Type:Journal Article
- Keywords:
GM-CSF;
cDNA;
Expression
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
One of the human GM-CSF cDNA was successfully isolated with a new screening method. Up 1/170 of positive rate was reached. The COS-7 cells were transfected with the cDNA clone and the resulting supernants contained colony-stimulatory activities. Human GM-CSF cDNA was also subcloned into a expression plasmid pKPL-2D and the unfused recombinant human GM-CSF was expressed in E. coli. The expressed products could maintain in vitro the growth of a cell line BMU- 2 and stimulate its colony formation. In addition, it could increase the colony formation of bone marrow cells from the patients with acute non-lymphatic leukemia (ANLL).
