DETECTION OF URINARY SPECIFIC IGM ANTIBODIES IN EPIDEMIC HEMORRHAGIC FEVER BY IGM ANTIBODY CAPTURE ELISA TECHNIQUE
- VernacularTitle:IgM抗体捕获ELISA法对流行性出血热患者尿液中特异性IgM抗体的检测
- Author:
Zhengwen LIU
- Publication Type:Journal Article
- Keywords:
hemorrhagic fever;
epidemic;
ELISA;
IgM, urine
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The specific IgM(SIgM) antibodies in 84 urine specimens from 52 patients with epidemic hemorrhagie fever (EHF) were detected by an IgM antibody capture ELISA technique with mixed monoclonal antibodies against multiple EHF virus strains. The total positive rate and the positive rate from day 7 to day 9 of illness were 72.62% and 83.87% respectively. The SIgM positive urine specimens oecured on day 3 of illness. The urine specimen, from healthy persons and the persons with other diseases were all negative. Therefore, high specificity and sensitivity were observed. No significant correlations were found both between the positive rates and drays of illness and between p(?)sitive rates and protein levels in urine specimens. The SIgM antibody titres were related with the days of illness rather than the protein levels in urine specimens. Furthermore, the positive rates were increased positively with the SIgM antibody titres.
