DETERMINATION OF CONJUGATED BILE ACIDS IN BILE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

VernacularTitle:高效液相色谱法测定胆汁中结合胆汁酸
Author: Yawen WANG
Publication Type:Journal Article
Keywords: chromatography; bile; conjugated bile acids; gallstone
From: Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(02):-
CountryChina
Language:Chinese
Abstract: A reversed-phase high performance liquid chromatographic method for the determination of conjugated bile acids in human bile had been reported. The procedure of sample preparation is simple since a guard column is used in this system. Recovery of the added bile salts was in the range of 87.01～110.75%. The limit of detection ranged from 0.09 ?g to 0.21 ?g. The method is used for the analysis of conjugated bile acids of bile from the patients with gallstone and control groups.