Responsiveness of staphylococcus aureus cowan strain activated human tonsillar B cells to human recombinant interleukin 4
- VernacularTitle:金黄色葡萄球菌Cowan株活化的人B细胞对人重组白细胞介素4的应答性
- Author:
Lei ZHENG
- Publication Type:Journal Article
- Keywords:
Recombinant interleukin 4;
Staphylococcus aureus cowan I;
Biological activity assay
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
The responsiveness of SAC-activated human B cells to human rIL-4 remains controversial. We have proved that rIL-4 definitely supports the proliferation of SAC-activated human tonsillar B cells. B cells preactivated with SAC for 24 hr or 72 hr exhibited the same magnitude of response to rIL-4. However, the 24 hr SAC-B cells showed substantially higher DNA synthesis without supply of rIL-4 than did of 72 hr SAC-B cells. The time that rIL-4 promoted proliferation of B cells is short. The growth factor activity of IL-4 for B cells is strongly unraveled in the first 24 hr cultivation and then, becoming weaker with prolonging of culture period. Two-step assay with 24 hr incubation for each step is introduced for the assessment of rIL-4 activity by SAC-B cells. Anti-? beads activated B cells showed better response to rIL-4 in comparison with SAC-B cells. B cells activated by either anti-? or SAC responded well to rIL-2 or rIL-4.
