Establishment of a long-term cytotoxic T cell line against syngeneic mouse leukemia and its biological characteristics
- VernacularTitle:同基因型肿瘤特异性T杀伤细胞系的建立及其生物学特性
- Author:
Wei CHEN
- Publication Type:Journal Article
- Keywords:
Mouse T lymphocytic leukemic cell clone;
Tumor specific cytotoxic T cells;
T cell growth factor(TCGF);
MHC restriction
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Cytotoxic T lymphocytes (CTL) derived from the spleen of SW-1 mice immunized in vivo with syngeneic mouse leukemia clonal cells LAC-1 were cultured in TCGF containing media and tested for cytotoxicity against LAC-1 cells in a routine ~(51)Cr-release assay. The experimental results showed that the CTL cell line was proved to be Thy 1 and Lyt 2, and therefore they were considered to be of T cell lineage. When CTL were expanded in the presence of TCGF, an augmented cytolytic activity in parallel with the increase of Thy 1 and Lyt 2 cells was shown. This cytotoxic T cell line had been maintained in a long-term culture for nearly 1 year and retained the initial reactivity. Specificity studies of the CTL showed that the cell line was highly specific for LAC-1 cells and its parent line L783 Leukemic cells and the cytolytic effect was H-2 restriction. The cytotoxicity of the CTL could be blocked by conventional or monoclonal antibodies against cell surface antigen (TSA) of LAC-1 cells. Antisera against H-2 antigens, shared by LAC-1 cells, also showed blocking activity.
