Quantitative Evaluation of Blood-Brain Barrier Permeability in Early-stage of Experimental Brain Injury
- VernacularTitle:实验性脑损伤早期血脑屏障通透性定量研究
- Author:
Ruxiang XU
- Publication Type:Journal Article
- Keywords:
blood-brain barrier;
brain edema;
brain injunies;
colloids
- From:
Chinese Journal of Trauma
1990;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
Fifty-seven rabbits were used for the experimental study of blood-brain barrier (BBB)permeability quantitatively in early stage of brain injury by a designated drop- ping weight method.Animals were divided into three groups:(1) control;(2)brain injury group; (3)therapeutic group.The brain injured animals were treated with antisodamine.Three different sizes of colloidal gold (OG)particles in diameter 5,10 and 15mu were given intravenously as tracers for quantitative investigation of BBB changes with light and electron microscope,respectively.In addition,brain water contents were de4rmined,The preliminary data indicated that the increase of BBB permeability began at 0.5h after trauma showing a few of 5 and 10 mu CG particles present in the endocytic pits and endothelial microvilli.Augmentation of BBB per meability reached its peak 6h after injury.Many 5,10 and 15mu CG tracers were seen penetrating the BBB through the openings at the tight junctions of endothelial cells or by vesicular transportation.The variation of brain water contents was closely correlated to the above mentioned BBB changes.The use of antisodamine resulted in a remarkable attanuation of BBB permeability and seemed advantageous in the treat- meat of cerebral edema after brain injury.
