ENZYME IMMUNOASSAY FOR SERUM THYROTROPIN RECEPTOR ANTIBODIES
- VernacularTitle:酶免疫法检测血清TSH受体抗体
- Author:
Hong ZHOU
- Publication Type:Journal Article
- Keywords:
EIA Thyrotropin receptor antibody Graves' disease
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
An enzyme immunoassay (EIA) for serum thyrotropin receptor antibodies (TRAb) was described. Polystyrene ball (3.2 mm in diameter) coated with solubilized guinea pig fat cell membranes (SGEM) was incubated with human serum and subsequently with horseradish peroxidase (HRP) conjugated staphylococcus protein A in a total volume of 50 ?I. The HRP activity was measured by spectrophotometer in a micro-cell with 3,3'5, 5'-tetramethylbenzydine (TMB) as substrate and 38 of 40 (95%) untreated Graves'patients (0.28?0.06, X?SD) and 15of 15 (100%) relapsed patients (0.25?0.06) had OD values at least 2 SD above normal subjects (0.09 ? 0.03), P
