A FLUOROMETRIC METHOD FOR THE ASSAY OF LENS ALDOSE REDUCTASE AND ITS APPLICATION IN DIABETIC RATS
- VernacularTitle:醛糖还原酶(AR)的荧光光度法测定及其在糖尿病大鼠晶体AR测定中的应用
- Author:
Yunping ZHOU
;
Jiaqing ZHENG
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus Aldose reductase Sorbitol pathway Aldose reductase inhibitor
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Rat lens aldose reductase (AR) activity was assayed in this study with a fiuorometric method. Rat lenses were homogenized in Na, K-phosphate buffer and centrifuged at 19127 ? g, 4℃ for 15 min. The supernatant was reserved as enzyme fraction. The incubation mixture contained 135 mmol KHaPO4-Na2HPO4 buffer (pH 7.0), 100 mmol Li2SO4, 6 ?mol NADPH, 1.0 mmol DL-glyceraldehyde or 10 mmol glucose and 20/ul enzyme fraction. AR activity in diabetic rat lent have not been shown to be significantly higher than that of the con rols. This method can be used properly to screen AR inhibitors in vitro.
