DETERMINATION OF GLUCOCORTICOID RECEPTORS IN HUMAN MONO-, POLY-MORPHONUCLEAR LEUKOCYTES, AND ITS SIGNIFICANCE
- VernacularTitle:人周围血单个核及多形核白细胞糖皮质激素受体的测定及其临床意义
- Author:
Zhimin LIU
- Publication Type:Journal Article
- Keywords:
Glucocorticoid Glucocorticoid receptor Leukocytes Cushing's syndrome Addison's disease Sheehan's syndrome
- From:
Chinese Journal of Endocrinology and Metabolism
1985;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
The method of glucocorticoid receptor (GR) in human peripheral mononuclear (MNL) and polymorphonucleas leukocytes (PML) was studied. The specific binding capacity of [3H] -Dex to leukocytes was estimated in 75 healthy subjects by radioligand binding method. 30 subjects were analysed by Scatchard plot to determine the maximal binding capacity (Ro) and dissociation constant (Kd), the Ro of MNL and PML were 5709 ? 901 and 6941 ?934 sites) cell respectively. Their Kd values were 4.83?1.21 and 5.78 ? 1.35 nmol/L respectively. The other 45 were investigated with the single point analysis, the specific binding capacity (Rs) of MNL and PML were 4462 ? 962 and 5483 ? 1146 sites/cell respectively. Coefficient of variation of this method in MNL and PML were 5.73?1.97 and 7.03?2.16% respectively. The results suggested that 1) no significant changes in the levels of GR in patients with Cushing's syndrome (Ro of MNL and PML were 6030?681 and 6885 ? 922 sites/cell respectively) and with Addison's disease (Ro were 4164 ? 564 and 5114 ? 658 sites/cell respectively); 2) the number of leukocytic GR was significantly lower in Shee-han's syndrome (Rs of MNL and PML were 3584?435 and 4131 ?562 sites/cell); 3) the number of GR was negatively correlated with the plasma cortisol concentration.
