PREPARATION OF MONOCLONAL ANTIBODIES AGAINST HUMAN VASCULAR ENDOTHELIAL CELLS AND THEIR POSSIBLE APPLICATION IN VASCULAR ENDOTHELIAL CELL TYPING
- VernacularTitle:抗人血管内皮细胞单克隆抗体的制备及其在血管内皮细胞分型上的可能应用
- Author:
Jieping CHEN
- Publication Type:Journal Article
- Keywords:
Vascular Endothelial Cell (VEC),Monoclonal Antibody.
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
Human vascular endothelial cells(VEC)were isolated from umbilicalyeins by trypsin digestion.Spleen cells from BALB/c mice having been im-munized with the isolated endothelial cells were fused with the mousemyeloma cell line SP2/0.After screening and subcloning,three differentmonoclonal antibodies(EC1,EC2,EC3)against human umbilical VEC wereobtained.These antibodies were proved to belong to immunoglobulin sub-classes(IgG_2,IgG_3,IgG_2).EC1 was shown to bind strongly to the vascularendothelial cell membrane,but not to react with peripheral lymphocytes andmonocytes in microcytotoxicity test.We also explored the possible applica-tion of the monoclonal antibodies in VEC typing.
