CALCIUM IONOPHORE STRONGLY INHIBITS THE GENERATION OF EFFECTOR KILLERS FROM PROLIFERATIVE CYTOTOXIC T CELLS
- VernacularTitle:钙离子导游剂强烈抑制杀伤T细胞的分化
- Author:
Weifeng CHEN
- Publication Type:Journal Article
- Keywords:
Phorbol Estor;
Calcium Ionophore;
Cortisone-Rresistant Thymocytes;
Cytotoxic T Cells;
Early T Cells.
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The Ly2~+ Cortisone-Resistant Thymocytes(CRT)were cultured in mediumcontaining IL2,CAS and various mitogens for 7 days.The cytolytic activityof effector CTL was assayed by ~(111)In-release cytotoxicity.At the K/T ratio32/1,the cytotoxicity of effector CTL generated from the stimulus of ConA,PMA.Calcium ionophore(CaI)and PMA+CaI was 46%,24%,13% and 5%respectively.This was the first time that the Cal showed a stronger effecton the inhibition of the generation of effector CTL than that of PMA.The inhibition of PMA and CaI on the differentiation of CTL could bepartially reversed by the removal of PMA and CaI-containing medium andreplaced with cytokine-containing fresh medium.The cytolytic activity wasincreased by three fold,but still 2.4 fold lower than that stimulated byConA at the same K/T ratio. The critical cytokine in the replaced medium was IL2 for the differentia-tion of CTL.Supplementation of IFNr,IL4 or CAS could not further increasethe cytotoxicity.However,the cytolytic activity was slightly increased whenIFNr was added at the initiation of culture. Early T cells could be induced to generate antigen-specific cytotoxic Tcells when cultured in the system with our designed protocol,
