STUDY ON OPTIMAL CONDITIONS OF LEUKOREGULIN INDUCTION BY USING ORTHOGONAL EXPERIMENTAL DESIGN
- VernacularTitle:白细胞调节素诱生条件的探讨
- Author:
Jian NI
;
Tianxing YE
;
Sikun YANG
- Publication Type:Journal Article
- Keywords:
Leukoregulin;
Anticancer lymphokine;
Orthogonal Experimental Design.
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Leukoregulin is an immunologic hormone whose anticancer action includethe inhibition of cellular proliferation and cytolysis of tumor cells eitherdirectly or indirectly by enhancing the tumor cell's susceptibility to dest-ruction mediated by natural killer cells.The optimal condition for the ind-uction of leukoregulin Were studied by using orthogonal experimental design.The optimal concentration is 1x10~7/ml for human spleen cells;3.1?g/mlfor phytohemagglutinin(PHA);12.5ng/ml for 12-0-tetradecanoyl-phorbol-13-acetate;15% for boyin serum.Leukoregulin activity prepared by 70 hincubation is maximum,the experiment also confirmed that the inducingleukoregulin effect of PHA-PⅢis significantly high.
