A MODIFIED METHOD OF DETERMINING NK ACTIVITY IN HUMAN PERIPHERAL BLOOD:WHOLE BLOOD ASSAY
- VernacularTitle:一种改良的人外周血NK活性检测法-全血法
- Author:
Beiqing LIU
;
Aihua BI
- Publication Type:Journal Article
- Keywords:
Natural Killer Activity;
Human Peripheral Whole Blood (WB);
Human Peripheral Blood Mononuclear cells (PBMC).
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
With use of ~(125)I-udR release test,a significant natural killer activityagainst K_(562) target cells has been determined in human peripheral wholeblood(WB).r There was a gradual decrease in NK activity as rise inthe dilution or WB.In comparison with peripheral monouclear cells(PBMC)oneself,the NK activity lowered distinctly at both 1:4 and 1:8 dilution ofWB(p0.4).It seems that neither the red blood cells nor thePlasma ffeect on NK activity of WB,The NK activity in WB as well as inPBMO was improved by incubation with human leukocyte interferon.TheNK activity of WB and PBMC in patients with hepatocellular carcinomawas signiticantly lower than in normal controls.The results showed tahtNK activity may be determined by using properly diluted WB instead orPBMC,and the assay can be easily performed in clinical practice.
