AN ANALYSIS OF LYMPHOCYTE SUBPOPULATIONS BY USING MONOCLONAL ANTIBODIES IN NON-OBESE DIABETIC MICE
- VernacularTitle:应用单克隆抗体对非肥胖糖尿病小鼠(NOD)淋巴细胞亚群分布的研究
- Author:
Changyu PAN
- Publication Type:Journal Article
- From:
Chinese Journal of Endocrinology and Metabolism
1985;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
To test if a lymphocyte subpopulation abnormality might be present at the onset of diabetes mellitus in new animal model of type 1 diabetes (NOD) and would serve as an early immunologic marker of the disease, the sequential changes, in peripheral lymphocyte subsets of 9female NOD and 11 ICR mice matched in age and sex were studied by using monoclonal antibodies. A significant increase in the proportion of Thy 1.2+(pan T) cells, Lyt 1+(helper /inducer) cells and Lyt 2+ (suppressor / cytotoxic) cells, but a significant decrease in Iak+ (B and activated T) cells and slight reduction of ratio of Lyt 1+/ Lyt 2+cells were noted. There was no tendency in these findings to change with increasing age of the mice and the changes of the lymphocyte subsets were not related to hyperglycemia. The results showed that not an abnormality of lymphocyte subsets could be used as a predictor for the onset of diabetes mellitus and the alterations in the proportion of T lymphocytes and their subsets could not account for the high incidence of diabetes mellitus nor could they be a prerequisite of T cell lymphopenia for the expression of DM gene(s) in NOD mice.
