ESTABLISHMENT AND INITIAL USE OF B_5 HYBRIDOMA CELL STRAIN SECRETING SPECIFIC MONOCLONAL ANTIBODY AGAINST THE HERPES SIMPLEX VIRUS-2
- VernacularTitle:分泌抗HSV—2型特异McAb的B_5杂交瘤细胞株的建立和初步应用
- Author:
Xinlong WANG
- Publication Type:Journal Article
- Keywords:
cell strain;
monoclonal antibody;
myeloma
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
A hybridoma cell strain secreting specific monoclonal antibody(McAb) against the herpes simplex virus-2(HSV-a) was established by fusing the mouse lmyeloma cell line SP 2/0-Ag 14 with the spleen cell of Balb/c mouse immunized by the HSV-2 Sav strain. The hybridoma cell strain can steadily secret McAb after being refrigarated for twenty months. The McAb titer in culture supernatants was 1: 120 and in mouse ascites was 10~(-7) by the indirect method ELISA. It is termed B_5 cell strain. The chromosome amount of B_5 cell was92-101; B_5 McAb was of IgGl subtype. It can react highly with HSV-2, and can not react with HSV-1, CMV, EHFV, Ad_3, RSV_(1387). Using the "microplate neutralization test", "Chick embryo chorioallantoic membrane neutralazation test" and "McAb for rabbit inoculated intracutaneously protectivity test", we demontrated that B_5 McAb has neutralization activity in vitro or in vivo (rabbit). 50% neutralization endpoint log is -1.7; B_5 McAb may react with fourteen of nineteen HSV-2 strains isolated from clinical patients. The indentification coincidence rate is 73.6% and can not react with five HSV-1 strains.
