Leukocyte Chemotaxis in Filter Assay and Its Affected Factors
- VernacularTitle:白细胞趋化性滤膜测定法及其影响因素
- Author:
Chengwu ZHANG
;
Weiwen ZOU
- Publication Type:Journal Article
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, polymorphonticlear leukocyte chemotaxis filter assay and its affected factors have been studied in accordance with the practical conditions.The results indicated that leukocyte chemotaxis may be detected by using Boycen chemotaxischamber prepared in our laboratory and filters made of mixed esters of cellulose athome.The appropriate experimental conditions are as follows:Single filter methodselected the filter of 3?m pore size.The cells were suspended in Eagle medium at aconcentration of 1?1.5/ml.The chamber was incubated at 37℃ in humidified airfor 120 min.Optimal concentration of bacterial culture filtration,zamsan activatedserum and lymphocyte derived chemotactic factor had eqeal cytotactic potency. Leukocyte chemotaxis is quantitated by counting the number of migrating cells atvarious depths within the filter.The mean number of migrating cells toward chemo-tactic factor is usually about twice as many as that of medium of control.Themodified method which may be acted as an index for investigating leukocyte functionin the experimental immunology and clinical research.
