Establishment of the mouse B cell mitogenic-and co-mitogenic monoclonal antibodies
- VernacularTitle:小鼠B淋巴细胞致有丝分裂原性——和协同致有丝分裂原性单克隆抗体的建立
- Author:
Deming SUN
- Publication Type:Journal Article
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
Sibirian hamster was immunized with mouse B myeloma cell(A20)lysate,and the im-munized spleen cells were fused with mouse myeloma cell line X63Ag8653.Four mono-clonal antibodies were thus obtained.While two(4B4 and 3C11)antibodies were shown to have strong direct mitogenic ac-tivity on mouse spleen B cells,all 4 antibodies could have synergistic effect in coope-rate with suboptimal doses of LPS to activate B cells.The mitogenic activity is ac-cessory cell independent.Four different surface molecules,as judged by both immuno-precipitating and binding assay,were compared.And the usefulness of these antibodiesin studying B cell activation and their functions were discussed.
