A BA-ELISA-Spot assay for the detection and enumeration of speifc antibody seretng cells
- VernacularTitle:用BA-ELISA斑点法检测特异的抗体分泌细胞
- Author:
Zhaojun LIU
;
Jiamin ZHOU
- Publication Type:Journal Article
- Keywords:
BA-ELISA-spot assay;
Antibody secreting cell;
Biotin-avidin.
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
A new solid-phase BA-ELISA-spot assay is described for detection and enumera-tion of antigen specific antibody secreting cells.Spleen cells from immunized mice areincubated in plstic wells precoated with specific an tigen.After removal of the cells,immobilized antibodies at the point of release from single cell are demonstrated bymeans of an immunenzyme procedure in which.enzyme-substrate(HRP-TMB)reactionsare performed.Blue spots are thus produced and counted with naked eye.
