Assessment of opsonophagocytic activity of specific hyperimmune products against pseumomonas aruginosa by using human neutrophil monolayers
- VernacularTitle:人体中性粒细胞单层粘附分离法检测抗绿脓杆菌特异免疫制剂的调理吞噬活性
- Author:
Shipeng WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
Opsonophagocytic actiyity of specific hyperimmune-plasma(PIP)and immunoglobulin(PIg)against Pseudomonas aruginosa was assessed by using human neutrophil monolay-ers.The result showed that specific hyperimmune plasma and immunoglobulin markedlyenhanced phagocytosis of autologous strain of Pseudomonas aruginosa by human neu-trophils when compared with normal plasma(Np)and immunoglobulin(Ng-3).When in-cubated with specific hyperimmune plasma(1:80)plus complement,the rate of phagocy-tosis was 68%,and with the specific immunoglobulins(PIg-1,PIg-2,and PIg-3,1:200),which were prepared by Ammonium sulfate,Rivanol,and ethyl alcohol precipitation,the rates of phagocytosis were 70.65%,26.35%,and 40.75% respectively in the pre-sence of complement.Among them,the specific immunoglobulin prepared by Ammoniumsulfate precipitation exerted the highest opsonic activity.In contrast,when incubatedwith normal plasma(1:80)or normal immunoglobulin(1:100)in the presence of com-plement,the rates of phagocytosis were only 18% and 17.9% respectively. Our study suggests that assessement of opsonophagocytic activity by.using neutro-phil monolayers appears to be a simple and useful method in the evaluation of immuneefficiency of specific immune plasma products against Pseudomonas aruginosa.
