Study on E receptor function-lysis of red blood cells by peripheral MNC
- VernacularTitle:E受体功能的探讨——外周血单个核细胞对红细胞的破坏作用
- Author:
Jing SHI
- Publication Type:Journal Article
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
Human and pig T lymphocytes have surface E receptors that can bind with SRBC or autoRBC forming E rosettes.The nature and significance of association of the E receptor with RBC have not yet been completely understood. There are two main approaches to study the significance of surface E receptor.Thefirst one is to study the change in lymphocyte functions induced by binding of surfaceE receptors with their ligands.The latter is to observe if some of SRBC or autoRBCbound with surface E receptors are actually damaged. In this study,the method for measuring SRBC and autoRBC lysis induced bycontact of the cells with pig peripheral mononuclear cells(MNC)was established andsome interesting results have been obtained.It was found that about 25% of autoRBC,82.6% of papain treated SRBC,61.5% of AET treated SRBC,and 20.2% of untreatedSRBC lysed at 5 hr.after they had bound with MNC.In all these tests MNC and RBCwere in the ratio of 1:1.When MNC were treated in 45℃ water bath for 1 hour toremove the E receptors o lysis of bound autoRBC was found. These results seem to suggest that pig MNC may initiate lysis of some of SRBC and autoRBC after they are bound to the MNC at room temperature for 5 hr,and this phenomenon may be utilized in the study of E receptors on the lymphocyte surface.
