A Simplified method to research allergen from Basophil level and its application in allergic asthma
- VernacularTitle:从嗜碱粒细胞水平研究过敏原的简易方法及其在过敏性哮喘的应用
- Author:
Yiping XU
- Publication Type:Journal Article
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
For improving the study of allergic asthma.We have designed a new method for assessing the degranulation of human basophils.The procedure involved the concentration of basophils with Ficoll-Hypaque(density 1.085).The staining of heparin whithin basophils with Alcian blue dye,and the counting of non degranulating basophils by using a haemacytometer.The percentage of basophils having degranulated in the presence of antigen over 30% was considered as positive.The HBDT was performed on 135Cases of allergic asthma and on 91 healthy Controls with Oermatophagoides farinaeextract as antigen.The positive rates were 68.2% in the above patients and 0% in thecontrols. Concordance of the results between HBDT and skin test,HBDT and ELISA,HBDTand RAST reached 83.3%,78.31% and 84% respectively.127 asthmatics were divided atrandom into two groups,with 63 cases in the treated by mite-desensitization.and 64cases for control. It was demonstrated that the treated group with positive HBDT showed the mosteffective results
