Solid-phase luminescence immunoassay of alphafetoprotein
- VernacularTitle:甲种胎儿蛋白的固相发光免疫测定
- Author:
Jia CHEN
- Publication Type:Journal Article
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
In this paper,a method of solid-phase luminescence immunoassay(LIA)of human alpha-fetoprotein(AFP)is described.The IgG of horse anti-human AFP-IgG was labelled with 6[N-(4-aminobutyl)-N-enthyl]-amino 2,3-dihydrophthalazine 1,4-dione (ABEI).The sensitivity of the method was 6.25ng/ml.The intra and inter coefficientsof variation was 7.9% and 9.8% respectively.The value of AFP of 20 patients assayedby LIA was compared with that assayed by radioimmunoassay(RIA).Although,the datafrom LIA were lower than that assayed by RIA,the results obtained by two methodscorrelated fairly well with a correlation coefficient(r)of 0.95.
