The study of monokines-The measurement of IL-1 from the macrophages activatetd by different agents
- VernacularTitle:单核因子(monokine)的研究——不同活化状态??培养上清液IL-1水平的比较
- Author:
Guifeng DING
- Publication Type:Journal Article
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
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Abstract:
In the present work we measured the concentration of Interleukin 1(IL-l) in the supernatant of cultured macrophage by the amount of ~3H-thymidine incoporated into T cells. The concentration of IL-1 was found to be marketly increased when macrophages were cultured with LPS (10?g/ml), SiO_2(100?g/ml), C.Parvum(1mg/mouse) Comparing the effects of these three stimulants on the incease of IL-1 from macrophage SiO_2 appeared to be the strongest one and C.P. and LPS were found to have cooperation for this increase in IL-1. Matrine,at the concentration of 100?g/ml, was found to antagonize the effect of LPS on theactivation of macrophage.
