The Study of hcG-McAb Kit For Pregnant Diagnosis The Characterization of 4 hcG McAb
- VernacularTitle:单克隆抗体妊娠诊断试剂的研制——四种hcG McAb的特性分析
- Author:
Rongfang ZENG
- Publication Type:Journal Article
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
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Abstract:
In this paper, 4 cell lines of hybridomas secreting hCG McAb were established & the charaterization of these McAb was analysed by RIA & ELISA.All of these McAb are specific for ?-hCG and can also be recognized by the intact hCG antigen. C_4 McAb is the highest specifieitg & with low cross-reaction to LH & FSH by competition test.It was found there were at least two epitopes against antigen among these four McAbs.Between C_3 & C_4 McAb there were observed high co-operation effect beside their obviously different epitopes against hCG antigen.So, these two McAb can be used to prepare two-site sandwich ELISA kit (one-step) for diagnosis. Eighty-one samples (including urine & blood) from early pregnant females were preliminarily detected by this hCG-McAb ELISA kit, the satisfactory results were obtained.
