Several Pseudomonas aeruginosa-LPS-containing Preparations and Their Protection against Pseudomonas aeruginosa Infection
- VernacularTitle:对几种含绿脓杆菌脂多糖的物质及其抗感染保护作用的初步观察
- Author:
Shiyu DU
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Lipopolysaccharide;
Vaccine
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Nine sorts of the preparations containing P.aeruginosa LPS were,With hot phenol- water method of Westphal,extracted from the cells or the cultures of P.aeruginosa (strain 017 of serotype 2 and its dissociative strains of both La.form and Sm.form), of which some cells or cultures had been pretreated respectively through homogenating, ultrasonic wave,autoclaving at 120c,and autolysis. The LPS preparations from Sm.from strain have higher contents of both KDO (2-Keto-3-Deoxyoctonic acid)and hexose than the rest do.Each of nine vaccines proved to be protecting mice against the challenge of strain 017 of over 10 LD_(?). The preparation extracted from the cultures having been pretreated through auto- claving at 12oC for 90 rain and ultrasonic wave shows the infrared absorption spectrum with decreased characteristic absorption peaks of lipopolysaccharide,but possesses the similar protective effect like the rest and otherwise lower toxicity.It indic-ates that the immuno comp etent substance is quite a heat-stable.
