Establishment of an ELISA System Using Monoclonal Antibodies forthe Measurement of Human Serum IgE——Evaluation of the Correlativity between McAb-and PcAb ELISA Systems and the Experimental Analysis of the Difference in Serum IgE Levels Detected
- VernacularTitle:应用抗人IgE的单克隆抗体测定人血清总IgE的ELISA方法建立——McAb与PcAb系统相关性测定及对造成两者测定值差异原因的实验分析
- Author:
Gang QIU
- Publication Type:Journal Article
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
In the initial experiment for the evaluation of the correlativity between McAb-and PcAb-ELISA systemes, we found that the serum IgE levels detected by the McAb-ELISA were about 10 times lower than those detected by the PcAb-ELISA system. Subsequent works demonstrated that this is due to the cross-reaction of PcAb with some non-IgE component(s) in human serum whereas the the possibility that the epitopes directed by our McAbs are not universally present on all IgE molecules, although ean not be definitely excluded at the present time, may not be responsible to the differences.
