A STUDY ON INDIRECT PEROXIDASE ANTIBODY TEST OF DETECTING IgM ANTIBODIES TO EPIDEMIC HEMORRHAGIC FEVER
- VernacularTitle:免疫酶染IgM检测法早期诊断流行性出血热的研究
- Author:
Tianmao QI
- Publication Type:Journal Article
- Keywords:
IGM antibody;
immunoperoxidase technics;
mercaptoethanol
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
The immunoporoxidase staining technics (IPST) was used in 37 patients with epidemic hemorrhagic fever (EHF) for detecting the specific IgM antibodies to EHF and compared with the method of IFA-IgG. The sera obtained at the early stage of the disease were ana- lyzed. In two cases, the results were negative by using the method of IPA-IgM antibodies. In 35 cases, the results were significantly higher than those by means of the method of IFA-IgG. But, the sera obtained at the late stage showed no difference between the two methods. The inhibition test and 2-ME tolerance tcst showed that the antibodics detected by the IPST technics were specific IgM ones, which could be found in the patient's serum on the second day of onset. The results showed that the IPA-IgM method is sensitive and highspecific and the results can be read under the light microscope. Thus, this method is convenient for the country doctors to use.
