Studies of Receptor of Concanavalin A on Mouse Thymocytes Quantitative Analysis by Fluorescence Method
- VernacularTitle:小鼠胸腺细胞刀豆素A受体研究——受体的荧光定量分析
- Author:
Jinrui WANG
- Publication Type:Journal Article
- Keywords:
concanavolin A, receptor, quantitative analysis, fluorescence, thyomocyte
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
By use of FITC-Con A made in China, the quantity of receptors on mouse thymocytes was determinated by saturation method and its affinity ocnstant was estimated. Afted the conditions of quantitative analysis with fluorescence method were defined, the quantity of receptors on thymocytes from 12 mice by two lots was carried out. The result demonstrated that the quantity of receptors per mouse thymocyte was 1.82?10~7 and its affinity constant K was 4.56?10~5M~(-1). This result was similar to radio-quantitative analysis of receptors, but without the disadvantage of radio-active contamination.
