Establishment of Sandwich ELISA for the Detection of Free-Form E-Receptors in Human Serum
- VernacularTitle:建立双抗体夹心酶标法(ELISA)检测人血清中游离E受体的研究
- Author:
Shilian LIU
- Publication Type:Journal Article
- Keywords:
ELISA, Free-Form E-Receptors, Human serum
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
Establishment of a Sandwich ELISA for detection of free-form E-receptors in human serum is reported.The specificity of this method was confirmed with inhibition test and the coefficient variance was 9.4～14.7％,indicating good reproducibility of the assay.The optimal conditions for the assay were:sheep anti-E-receptor IgG at 15?g/ml for coating polystyrene plates, HRP-IgG conjugate at a dilution of 1:2,000.110 normal human sera and 140 cord blood sera were tested and all showed satisfactory results, The concentrations of E-receptors in normal sera and cord blood sera were 18?2?g/ml and 5?1?g/ml respectively(p
