The Improvements on the Determination Method And Data Normalization of Specific IgE
- VernacularTitle:对于特异性IgE测定方法及数据校正的改进
- Author:
Jinfang WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
A Computer-normalized micro-ELISA assay with some improvements to reduce non -specific binding for determination of Dermatophagoides farinae-specific IgE is desc- ribed.Bovine serum albumin was added to the conjugate buffer and serum dliuent to re- duce“noise”often encountered in ELISA assay,resulting in decreased background and increased sensitivity for determining specific IgE.An“IP”milliunit system was adopted to communicate the results of the assay in order that the clinician can compare them more easily.A computer programme for standardization of the results was developed. The automation and high efficiency of computer allowed a reliable and much more ra- pid management of all the experiment data.Interassay coefficients declined from 15.81% into 8.20% after normalization.This computerized normalization method can also be used in other allergen-specific IgE determinations,employing the same progra- mme.The specificity of this experiment was confirmed by absorption studies.
