A Study on the Detection of Circulating ImmuneComplexes in Mice with Raji Cell Enzyme-linkedImmunosorbent Assay(Raji一ELISA)
- VernacularTitle:用Raji细胞酶联免疫吸附试验测定小鼠循环免疫复合物的探讨
- Author:
Guoqing LIU
- Publication Type:Journal Article
- Keywords:
Raji-ELISA;
Immune complexes
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
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Abstract:
This paper reports a method for detecting circulating immune complexes (CIC) inmice with an enzyme-linked immunosorbent assay (Raji-ELISA). This method isspecific, sensitive and easy to perform, the reagents are safe and reliable, and it canbe used without much difficulty. It has a sensitivity of 5.5?g/ml, along with a goodspecificity and reproducibility. Good correlation was observed between the Raji-ELISA and polyethylene glycol (PEG) precipitation assay. Forty-six serum samplesfrom normal Kunming mice were determined by the Raji-ELISA assay, and theconcentration of CIC was 26.18?20.41?g/ml. Only 6.5% of normal Kunming miceshowed a value of CIC above + 2s. The mice allografted with spleen cells fromnormal mice had no elevation in the concentration of CIC. However, the CIC ofEhrlich ascite tumor bearing mice showed a great increase, reached a value of105.12 + 38.76?g/ml with a total positivity of 93%.
