Study of Antigenicity of AFP Using Monoclonal Antibodies Specific for Human AFP
- VernacularTitle:应用抗甲胎蛋白单克隆抗体对AFP抗原性的研究
- Author:
Zhiqing HU
;
Hanyi YANG
;
Shourou LI
- Publication Type:Journal Article
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Abstract-Eleven mouse monoclonal antibodies (McAbS)specific for human alpha-fetopr-otein(AFP) have been produced by the hybridoma technique. Immunodiffusion was us-ed for determining the immunoglobulin class and subclass of the McAbS. Seven of them are IgG; two are IgM. At least 4 different antigenic determinants on human AFPcan be recognized by using the McAbS and cross-two-site sandwich solid radioimmun-oassay. One of the McAbS can specially react with one of the fragments of AFP dig-ested by pepsin. In addition, the cross-reactivity among the AFPs of different mam-malian species was also tested by using the McAbS.
