Content Determination of Astragaloside Ⅳ in Qizhi Jiangtang Granules by HPLC-ELSD
- VernacularTitle:HPLC-ELSD法测定芪蛭降糖颗粒中黄芪甲苷的含量
- Author:
Qiuyue LI
;
Yixuan ZHANG
;
Yue SHI
- Publication Type:Journal Article
- Keywords:
HPLC-ELSD;
Qizhi jiangtang granules;
Astragaloside Ⅳ
- From:
China Pharmacy
2007;0(32):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish HPLC-ELSD method for content determination of astragaloside Ⅳ in Qizhi jiangtang granules. METHODS:The separation was performed on Hypersil ODS2(250 mm?4.6 mm,5 ?m)column with acetonitrile-water(32 ∶ 68)as mobile phase. The flow rate was 1.0 mL?min-1. Injection volume was 10 ?L. The column temperature was 25 ℃. The temperature of drift tube for measurement was 110 ℃ and flow rate of N2 was 3.12 SLPM. RESULTS:Regression equation was A=1.699 C+6.181. The linear range of astragaloside Ⅳ was 0.259～1.295 mg?mL-1(r=0.999 6,n=5) with lowest detectable limit of 5.18 ?g?mL-1. An average recovery rate were 97.75%(RSD=2.17%). CONCLUSION:The method is simple,rapid and reliable for quality control of Qizhi jiangtang granules.
