Content Determination of Oxaliplatin in Oxaliplatin Liposome by HPLC
- VernacularTitle:HPLC法测定奥沙利铂脂质体中奥沙利铂的含量
- Author:
Jing ZHAO
;
Hua ZHAO
;
Xinhui JIANG
- Publication Type:Journal Article
- Keywords:
Oxaliplatin;
Liposome;
HPLC;
Content
- From:
China Pharmacy
2007;0(32):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for the content determination of oxaliplatin in Oxaliplatin liposome. METHODS:The separation was performed on Hypersil C18(250 mm?4.6 mm,5 ?m) column with methanol-water(5 ∶ 95,V/V) as mobile phase at flow rate of 1.0 mL?min-1. The detection wavelength was set at 250 nm and column temperature was 30 ℃. RESULTS:The linear range of oxaliplatin was 12.2～122.0 ?g.mL-1(n=5,r=0.999 9) with an average recovery of 98.58%(RSD=1.83%). The limit of detection(LOD) was 97.6 ng?mL-1. CONCLUSION:The method is simple,sensitive and accurate for the content determination of oxaliplatin in Oxaliplatin liposome.
