Content Determination of Baicalin in Xiaoer Zhike Granule by HPLC
- VernacularTitle:HPLC法测定小儿止咳颗粒中黄芩苷的含量
- Author:
Weiming LIAO
;
Guizhen LIU
;
Guobo ZHOU
- Publication Type:Journal Article
- Keywords:
Xiaoer zhike granule;
HPLC;
Baicalin;
Content determination
- From:
China Pharmacy
2007;0(31):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish the HPLC method for the content determination of baicalin in Xiaoer zhike granule. METHODS: YWG C18 (150 mm?4.6 mm,10 ?m) column was used and the mobile phase consisted of methnol-water-phosphoric acid (47 ∶ 53 ∶ 0.2) with the flow rate of 2.5 mL?min-1. The detection wavelength was set at 280 nm and injection volume was 10 ?L. RESULTS: The linear range of baicalin was 25～200 ?g?mL-1(r=0.999 6) with an average recovery of 99.57%(RSD=1.9%,n=6). CONCLUSION: This method is sensitive, simple and accurate for the quality control of Xiaoer zhike granule.
