Effect of Huoxue Jiedu Recipe Medicated Serum on Proliferation and Cell Cycle Status of Keratinocytes of Psoriasis
- VernacularTitle:活血解毒方含药血清对银屑病角质形成细胞增殖及细胞周期的影响
- Author:
Junwei WANG
;
Min LI
;
Ruijie ZHAI
;
Yuhe YAN
;
Wali LIU
- Publication Type:Journal Article
- Keywords:
Huoxue Jiedu Recipe(Herbal recipe for activating blood and removing toxins);
Psoriasis;
Keratinocytes;
Cell proliferation;
Cell cycle
- From:
Journal of Traditional Chinese Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Huoxue Jiedu Recipe(Herbal recipe for activating blood and removing toxins)on proliferation and cell cycle status of keratinocytes cultured in vitro in psoriasis.Methods Using the serum pharmacologic technique,the clean SD rats were adopted to make the Huoxue Jiedu Recipe medicated serum(high,moderate,low dose),Dexamethasone medicated serum,and blank serum,which were added into the keratinocytes culture system respectively.After incubated for 48 hours,the cell proliferation of keratinocytes was measured by MTT assay,and the cell cycle distribution estimated with flow cytometer(FCM).Results The different density of Huoxue Jiedu Recipe could all inhibit significantly the generation of COLO-16 keratinocyte line and change the distribution proportion of each phase in the cell division cycle.Conclusion Huoxue Jiedu Recipe treats psoriasis through inhibiting the proliferation of keratinocytes.
