Effect of PTTG on the growth and fluorouracil sensitivity of cholangiocarcinoma cell Line QBC939
- VernacularTitle:PTTG对胆管癌细胞生长和5-FU敏感性影响的研究
- Author:
Ling CHEN
- Publication Type:Journal Article
- Keywords:
Bile Duct Neoplasms/gene;
Fluorouracil;
PTTG;
Tumor Cells,Cultured
- From:
Chinese Journal of General Surgery
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effct of PTTG gene on the growth and sensitivity to 5-FU of cholangiocarcinoma cell.Methods The pcDNA3.1-PTTGas that contained full-length antisense PTTG,and pcDNA3.1(+) was transfected into the cholangiocarcinoma cell line QBC939.We successfully established and identified cell sublines tQBC939(PTTG-),tQBC939(pcDNA3.1).Cell growth curve was mapped out,cell proliferation rate was assessed by MTT assay and cell cycle percentage was counted by flow cytometry.After treatment with 5-FU,cell survival rate was assessed by MTT assay and IC50 was calculated.Flow cytometry and Hoechst staining were used to demonstrate discrepancy of cell apoptosis rates.Results Compared with other two groups,tQBC939(PTTG-) grew more rapidly and had a higher proportion of cells in S phase,whereas less cells in G2/M phase(P
