Study of siRNA targeting survivin on inducing sensitization of heaptocarcinoma cells to chemotherapy
- VernacularTitle:靶向survivin的siRNA诱导肝癌细胞化疗敏感性的研究
- Author:
Xin LU
;
Qichang ZHENG
- Publication Type:Journal Article
- Keywords:
Liver Neoplasms;
Survivin Gene;
siRNA;
Mitomycine;
Sensitiveity
- From:
Chinese Journal of General Surgery
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of siRNA targeting survivin on chemotherapy sensitivity of HCC cells.Methods siRNA eukaryotic expression vector was generated.After the vector stablely transfected into HepG2 cells,the effects of chemotherapy drugs on HCC cells were observed.Results The recombinant plasmid Psilence(+)-survivin was successfully constructed.Survivin mRNA expression inhibition ratio reached 73% detected by RT-PCR.MTT methods detected that siRNA treated HCC cells were affected by MCC,the survival rate of HepG2,HepG2/Silence(-) cells was inhibited only at 48 h(0.505?0.015) compared to control groups untreated with MCC(0.824?0.322)(P
