Construction and identificication of siRNA recombinant expression vector targeting HIF-1? gene
- VernacularTitle:缺氧诱导因子-1?靶向siRNA重组表达载体的构建和鉴定
- Author:
Yingchun HU
;
Chengyou WANG
;
Yongqiang ZHANG
;
Yong NI
- Publication Type:Journal Article
- Keywords:
HIF-1?;
RNA interference;
Gene Therapy
- From:
Chinese Journal of General Surgery
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct siRNA recombinant expression vector targeting HIF-1? gene.Methods Genome sequences of HIF-1? gene were retrieved from gene bank,and four pairs of oligonucleotides were synthesized and inserted into plasmid pGensil-1.Then,DH5? strains were transformed,and plasma was extracted and identified by restriction endonuclease and sequence analysis.Results It was confirmed by sequence analysis that siRNA recombinant expression vector targeting HIF-1? gene coincided completely with the designs.Conclusions The siRNA recombinant expression vector targeting HIF-1? gene has been constructed successfully and lays a foundation for furher study on the biological behavior of tumors.
