Study on the Preparation of Buserelin Acetate Nanoparticles and Its Release Property in Vitro
- VernacularTitle:醋酸布舍瑞林纳米粒的制备及体外释放特性研究
- Author:
Lei HU
;
Songqing LIU
;
Qing DAI
- Publication Type:Journal Article
- Keywords:
Buserelin acetate;
Nanoparticle;
Double emulsion method;
In vitro release rate
- From:
China Pharmacy
2007;0(29):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To prepare Buserelin acetate nanoparticles(BA-NP) and to investigate its release property in vitro.METHODS: The BA-NP was prepared using double emulsion method.The content determination of BA-NP was performed using HPLC,and encapsulation efficiency and drug-loading rate of BA-NP were calculated.In vitro drug release property of nanoparticles was investigated by bag filter method.RESULTS: Prepared nanoparticles were even and regular in appearance.The linear range of buserelin acetate was 0.1～8.0 ?g?mL-1(r=0.999 9) with an average recovery of 105.38%.The RSD of intra-day and inter-day were lower than 1.78% and 0.93% respectively.The encapsulation efficiency of nanoparticles was(63.37?0.29)% and drug-loading rate of(1.03?0.09)%.The accumulative release rate of nanoparticles in phosphate buffer(pH=7.4) at 72 h was 62.35%.CONCLUSION: The preparation process of BA-NP is simple and particle with ideal release effect.
