Study on Fragmentation Pathways of Amoxicillin by Electrospray Ionization Multi-stage Tandem Mass Spectrometry
- VernacularTitle:电喷雾串联质谱法研究阿莫西林的裂解方式
- Author:
Hongsen ZHANG
- Publication Type:Journal Article
- Keywords:
Amoxicillin;
Electrospray ionization multi-stage tandem mass spectrometer;
Fragmentation pathway;
Ion fragment
- From:
China Pharmacy
2007;0(29):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate fragmentation pathways of amoxicillin using electrospray ionization multi-stage tandem mass spectrometry,and to provide reference for quality and quantity analysis of amoxicillin.METHODS: Amoxicillin was further purified by high performance liquid chromatography,and the fragmentation mechanisms were investigated by electrospray ionization multi-stage tandem mass spectrometer.RESULTS: The ?-lactam ring of amoxicillin has three different opening modes;2 kinds of different m/z:114 ion fragments are obtained by two different fragmentation pathways;m/z:349 ion fragments can be a characteristic fragment of amoxicillin for quality and quantity analysis of amoxicillin.CONCLUSION: The method is sensitive and accurate for further study of transformation and metabolism of amoxicillin and its quality and quantity analysis.
