Content Determination of 5 Kinds of Amino Acids in Compound ?-Ketoacid Tablets by HPLC after Pre-column Programming Derivatization
- VernacularTitle:HPLC柱前程序衍生法测定复方?-酮酸片中5种氨基酸的含量
- Author:
Gengshen SONG
;
Jianguo JIANG
;
Ting SUN
- Publication Type:Journal Article
- Keywords:
HPLC;
Programming derivatization;
Compound ?-ketoacid tablets;
Amino acids;
Content
- From:
China Pharmacy
2007;0(29):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an HPLC method with pre-column programming derivatization for the content determination of 5 kinds of amino acids in Compound ?-ketoacid tablets.METHODS: The separation was performed on C8 column(gradient elution) with column temperature at 40 ℃.OPA was used as the derivation reagent.The detection wavelength was set at 338 nm.The contents of L-lysine acetate,L-threoine,L-tryptophan,L-histidine,L-tyrosine in Compound ?-ketoacid tablets were determined.RESULTS: The linear ranges of L-lysine acetate,L-threoine,L-tryptophan,L-histidine,L-tyrosine were 0.10～5.25 mg?mL-1(r=0.999 9),0.052～2.65 mg?mL-1(r=0.999 8),0.022～1.15 mg?mL-1(r=0.999 9),0.038～1.91 mg?mL-1(r=0.999 7),0.030～1.51 mg?mL-1(r=1.000 0),respectively.The average recovery was 99.8%～100.1%(RSD=0.1%).CONCLUSION: This method is exclusive,sensitive and reliable for the content determination of amino acids in Compound ?-ketoacid tablets.
