Quality Investigation of Shuanghuanglian Oral Solutions from Different Manufactures
- VernacularTitle:不同厂家双黄连口服液的质量考察
- Author:
Zude LIU
- Publication Type:Journal Article
- Keywords:
Shuanghuaglian oral solutions;
HPLC;
Baicalin;
Chlorogenic acid;
Forsythin
- From:
China Pharmacy
2007;0(28):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To study the quality of Shuanghuanglian oral solutions from different manufactures. METHODS: The contents of baicalin, chlorogenic acid and forsythin in Shuanghuanglian oral solution on the market were determined by HPLC. The determination was performed on Agilent ZORBAX 80A Extend-C18 (150 mm?4.6 mm,5 ?m) column with column temperature set at 30 ℃. The mobile phase for baicalin consisted of methanol-water-acetic acid (50 ∶ 50 ∶ 1, V/V) at flow rate of 1.0 mL?min-1 and detection wavelength of 274 nm. The mobile phase for chlorogenic acid consisted of methanol-water-acetic acid (20 ∶ 80 ∶ 1, V/V) at flow rate of 0.8 mL?min-1 and detection wavelength of 324 nm. The mobile phase for forsythin consisted of acetonifrile-water (25 ∶ 75, V/V) at flow rate of 1.0 mL?min-1 and detection wavelength of 278 nm. RESULTS: Shuanghuanglian oral solutions from different manufactures conformed to the requirements of the first column of Chinese Pharmacopeia (2005 edition). The content differences of chlorogenic acid in Shuanghuanglian oral solutions were almost 3 times, which may be the cause of the difference of clinical efficacy. CONCLUSION: The minimum detection limit only has been established for the quality standard of Shuanghuanglian oral solution. It is suggested to improve the quality standard of Shuanghuanglian oral solution.
